Huang L S, Jaeger J S, Usher D C
J Lipid Res. 1983 Nov;24(11):1485-93.
Western blot analysis of the alloantisera (i.e., anti-Lpq1, anti-Lpq2, anti-Lpq3, and anti-Lpq4) which defined the three lpq genes of rabbit linkage group VIII showed that they reacted strongly with an apolipoprotein of molecular weight 320,000. They also cross-reacted with an apolipoprotein of molecular weight 220,000. The two apolipoproteins that reacted with the alloantisera were found by SDS-polyacrylamide gel electrophoresis to be present in very low density (VLDL), intermediate density (IDL), and low density (LDL) lipoprotein fractions and by Western blot analysis to react with an anti-apolipoprotein B antiserum. These results support the conclusion that the alloantisera react with allotypes associated with the B apolipoproteins. The distribution of the four allotypes among different lipoprotein fractions, however, differed. The quantitative competitive Enzyme Linked Immunosorbant Assay (ELISA) showed that the Lpq1, Lpq2, and Lpq4 allotypes were found in the highest concentration in VLDL, IDL, and LDL, and in significantly lower concentrations in plasma chylomicrons. The concentrations of these allotypes in high density lipoproteins (HDL) as measured in the ELISA were about 1% of the concentrations found in LDL. The Lpq3 allotype, on the other hand, was present in the highest concentrations only in IDL and LDL and in significantly lower concentrations in VLDL and plasma chylomicrons. Surprisingly, the concentration of the Lpq3 allotype in HDL was 20% of the level found in LDL.
对确定家兔连锁群VIII的三个lpq基因的同种抗血清（即抗Lpq1、抗Lpq2、抗Lpq3和抗Lpq4）进行的蛋白质免疫印迹分析表明，它们与一种分子量为320,000的载脂蛋白发生强烈反应。它们还与一种分子量为220,000的载脂蛋白发生交叉反应。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳发现，与同种抗血清发生反应的这两种载脂蛋白存在于极低密度（VLDL）、中间密度（IDL）和低密度（LDL）脂蛋白组分中，并且通过蛋白质免疫印迹分析发现它们与抗载脂蛋白B抗血清发生反应。这些结果支持了同种抗血清与B载脂蛋白相关的同种异型发生反应这一结论。然而，四种同种异型在不同脂蛋白组分中的分布有所不同。定量竞争性酶联免疫吸附测定（ELISA）表明，Lpq1、Lpq2和Lpq4同种异型在VLDL、IDL和LDL中的浓度最高，而在血浆乳糜微粒中的浓度显著较低。ELISA测定的这些同种异型在高密度脂蛋白（HDL）中的浓度约为在LDL中发现浓度的1%。另一方面，Lpq3同种异型仅在IDL和LDL中浓度最高，而在VLDL和血浆乳糜微粒中的浓度显著较低。令人惊讶的是，Lpq3同种异型在HDL中的浓度是在LDL中发现水平的20%。
