Lindenbaum M H, Livett B G
Muscle Nerve. 1983 Nov-Dec;6(9):638-45. doi: 10.1002/mus.880060904.
Acetylcholinesterase (AChE) was extracted from normal and dystrophic C57BL/6J mouse hindlimb muscles and its molecular forms fractionated by sucrose density gradient ultracentrifugation. In the soleus muscles from 6- to 7-week-old mice an increase in the 3 Svedberg unit (S) and a decrease in the 16S AChE molecular forms was observed in dystrophic animals compared to controls. At 12-13 weeks of age, no major significant differences in the relative proportions of AChE molecular forms were noted. In the extensor digitorum longus (EDL) muscles of 6- to 7-week-old dystrophic mice a significant decrease in the proportion of the 10S AChE molecular form and an increase in the 3S and 5S forms was observed. At 12-13 weeks, the dystrophic EDL muscles again displayed a decrease in the 10S form; however, the increase in the 3S and 5S AChE forms, while still apparent, was not significant. These results provide evidence for a biochemical abnormality in the distribution of specific AChE molecular forms, and a differential expression of this abnormality in the soleus and EDL muscles.
从正常和营养不良的C57BL/6J小鼠后肢肌肉中提取乙酰胆碱酯酶（AChE），并通过蔗糖密度梯度超速离心法对其分子形式进行分级分离。在6至7周龄小鼠的比目鱼肌中，与对照组相比，营养不良动物的3沉降单位（S）增加，16S AChE分子形式减少。在12至13周龄时，AChE分子形式的相对比例未发现重大显著差异。在6至7周龄营养不良小鼠的趾长伸肌（EDL）中，观察到10S AChE分子形式的比例显著降低，3S和5S形式增加。在12至13周时，营养不良的EDL肌肉中10S形式再次减少；然而，3S和5S AChE形式的增加虽然仍然明显，但并不显著。这些结果为特定AChE分子形式分布的生化异常以及比目鱼肌和EDL肌肉中这种异常的差异表达提供了证据。
