Stefanovich V
Neurochem Res. 1983 Nov;8(11):1459-69. doi: 10.1007/BF00965001.
The uptake of adenosine is studied in microvessels isolated from a bovine cortex. The KM value for adenosine uptake is 1.92 microM and the Vmax is 1.93 picomole/mg protein/10 min. This high affinity uptake system is very sensitive to inhibition by dipyridamole and papaverine. The uptake of adenosine by microvessels is also inhibited by CuCl2 and by high concentration (2 mM) of adenine nucleotides. Using a series of four xanthines is observed that the adenosine uptake system is most inhibited by 3-methyl-l-(5'-oxohexyl)-7-propylxanthine and the least by caffeine. Theophylline causes a stimulation of adenosine uptake by microvessels. The results obtained agree with the existence of the nucleoside transport system associated with the blood-brain barrier, as previously observed by in vivo studies and experiments with rat brain capillaries.
对从牛大脑皮层分离出的微血管中的腺苷摄取情况进行了研究。腺苷摄取的米氏常数（KM）为1.92微摩尔，最大反应速度（Vmax）为1.93皮摩尔/毫克蛋白质/10分钟。这种高亲和力摄取系统对双嘧达莫和罂粟碱的抑制非常敏感。微血管对腺苷的摄取也受到氯化铜和高浓度（2毫摩尔）腺嘌呤核苷酸的抑制。使用一系列四种黄嘌呤观察到，腺苷摄取系统受3-甲基-1-（5'-氧代己基）-7-丙基黄嘌呤的抑制作用最强，受咖啡因的抑制作用最弱。茶碱会刺激微血管对腺苷的摄取。所获得的结果与先前在体内研究和大鼠脑毛细血管实验中观察到的与血脑屏障相关的核苷转运系统的存在情况相符。
