Barberis C, Minn A, Gayet J
J Neurochem. 1981 Feb;36(2):347-54. doi: 10.1111/j.1471-4159.1981.tb01601.x.
Kinetics for transport of adenosine into guinea-pig neocortex synaptosomes were studied by incubating them with [14C]adenosine for up to 30 s. The apparent Km value of the high-affinity transport system for adenosine was 21.1 microM and the Vmax value was 257.3 pmol/min/mg protein. The transport system was inhibited by both compounds structurally related (compounds 554 and 555) and unrelated (dipyridamole) to adenosine. Because electrically stimulated synaptosomes release up to 1.5% of the adenosine derivative content per min, the physiological significance of adenosine uptake is discussed as a possible mechanism to compensate for the loss of adenine nucleotides from synaptosomal preparations.
通过将豚鼠新皮质突触体与[14C]腺苷孵育长达30秒，研究了腺苷转运至豚鼠新皮质突触体的动力学。腺苷高亲和力转运系统的表观Km值为21.1微摩尔，Vmax值为257.3皮摩尔/分钟/毫克蛋白质。该转运系统受到与腺苷结构相关（化合物554和555）和不相关（双嘧达莫）的两种化合物的抑制。由于电刺激的突触体每分钟释放高达1.5%的腺苷衍生物含量，因此讨论了腺苷摄取的生理意义，作为一种可能补偿突触体制剂中腺嘌呤核苷酸损失的机制。
