腺苷在离体大鼠脑毛细血管中的摄取。

Uptake of adenosine by isolated rat brain capillaries.

作者信息

Wu P H, Phillis J W

出版信息

J Neurochem. 1982 Mar;38(3):687-90. doi: 10.1111/j.1471-4159.1982.tb08686.x.

DOI:10.1111/j.1471-4159.1982.tb08686.x

Abstract

Adenosine uptake by isolated rat brain capillaries is a carrier-mediated, temperature- and pH-sensitive process. The Km value for adenosine uptake is 4.74 microM and the Vmax is 21.7 picomol/mg protein/10 min. This is a high-affinity uptake system that can be cross-inhibited by several nucleosides and by the adenosine analogs tubercidin and 5'-deoxyadenosine. The uptake is very sensitive to inhibition by papaverine, hexobendine, and dipyridamole. These results confirm the existence of a nucleoside transport system associated with the blood-brain barrier observed during in vivo studies.

摘要

分离的大鼠脑毛细血管对腺苷的摄取是一个载体介导的、对温度和pH敏感的过程。腺苷摄取的Km值为4.74微摩尔，Vmax为21.7皮摩尔/毫克蛋白质/10分钟。这是一个高亲和力摄取系统，可被几种核苷以及腺苷类似物杀结核菌素和5'-脱氧腺苷交叉抑制。摄取对罂粟碱、己酮可可碱和双嘧达莫的抑制非常敏感。这些结果证实了体内研究中观察到的与血脑屏障相关的核苷转运系统的存在。