金黄色葡萄球菌核糖体RNA的“红霉素抗性”甲基化序列。
The "erythromycin-resistance" methylated sequence of Staphylococcus aureus ribosomal RNA.
作者信息
Ranzini A C, Dubin D T
出版信息
Plasmid. 1983 Nov;10(3):293-5. doi: 10.1016/0147-619x(83)90044-6.
We have determined the sequence of an oligonucleotide from the large ribosomal subunit RNA of Staphylococcus aureus whose methylation renders the organism resistant to erythromycin and other antibiotics (the "MLS" phenotype). Analysis of RNase A digests of [3H]methyl-, 32P-labeled RNA yielded the sequence GG . m6(2)A . AAGACp, where m6(2)A is an N6-dimethylated adenosine residue that in sensitive cells is unmethylated. Comparison with homologous sequences recently reported for Saccharomyces cerevesiae mitochondria indicates that an A to G mutation in this latter system mimics dimethylation in St. aureus with regard to functional consequences.
我们已经确定了来自金黄色葡萄球菌大核糖体亚基RNA的一段寡核苷酸序列，该序列的甲基化使该生物体对红霉素和其他抗生素产生抗性（“MLS”表型）。对[³H]甲基-、³²P标记的RNA进行核糖核酸酶A消化分析，得到序列GG.m⁶(2)A.AAGACp，其中m⁶(2)A是一个N⁶-二甲基化腺苷残基，在敏感细胞中该残基未被甲基化。与最近报道的酿酒酵母线粒体同源序列进行比较表明，在功能后果方面，后一个系统中的A到G突变模拟了金黄色葡萄球菌中的二甲基化。
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