Katz L, Brown D, Boris K, Tuan J
Department of Molecular Biology, Abbott Laboratories, Abbott Park, IL 60064.
Gene. 1987;55(2-3):319-25. doi: 10.1016/0378-1119(87)90291-5.
The ermE gene was cloned from Streptomyces erythraeus into Escherichia coli on a series of plasmids. When transcribed from the lac promoter, ermE conferred high-level resistance to erythromycin and other macrolide-lincosamide-streptogramin-B (MLS) antibiotics. A methylase activity capable of N6-mono- and N6,N6-dimethylation of adenine residues in E. coli rRNA was detected in extracts of MLS-resistant cells. In addition, rRNA extracted from MLS-resistant E. coli contained N6-mono- and N6,N6-dimethylated adenine residues.
ermE基因从红色链霉菌克隆到一系列质粒上，并导入大肠杆菌。当从乳糖启动子转录时，ermE赋予对红霉素和其他大环内酯-林可酰胺-链阳菌素B（MLS）抗生素的高水平抗性。在MLS抗性细胞提取物中检测到一种能够对大肠杆菌rRNA中的腺嘌呤残基进行N6-单甲基化和N6,N6-二甲基化的甲基化酶活性。此外，从MLS抗性大肠杆菌中提取的rRNA含有N6-单甲基化和N6,N6-二甲基化的腺嘌呤残基。
