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一种改进的菌落杂交方法，对单基因检测的灵敏度显著提高。

An improved colony hybridization method with significantly increased sensitivity for detection of single genes.

作者信息

Maas R

出版信息

Plasmid. 1983 Nov;10(3):296-8. doi: 10.1016/0147-619x(83)90045-8.

DOI:10.1016/0147-619x(83)90045-8
PMID:6657780
Abstract

By introducing a simple modification of existing methods, colony hybridization has been used to detect single copy genes coding for a heat-stable enterotoxin in wild-type strains isolated from patients with diarrhea. The modification described in this communication results in an approximately 100-fold increase in sensitivity, probably by increasing the total denatured plasmid DNA fixed to the paper.

摘要

通过对现有方法进行简单改进，菌落杂交已被用于检测从腹泻患者分离出的野生型菌株中编码热稳定肠毒素的单拷贝基因。本通讯中描述的改进使灵敏度提高了约100倍，这可能是通过增加固定在滤纸上的总变性质粒DNA实现的。

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