通过γ射线辐照提高乙二醇中大麦细胞核DNA的产量。

Increase of the yield of DNA from barley nuclei in ethylene glycol by gamma-irradiation.

作者信息

Naito T, Yamaguchi H

出版信息

Radioisotopes. 1983 Jul;32(7):333-5. doi: 10.3769/radioisotopes.32.7_333.

DOI:10.3769/radioisotopes.32.7_333

Abstract

After gamma-irradiating the nuclei isolated from dry seed-embryo of barley in the presence of varying concentrations of ethylene glycol, macromolecular DNA (deoxyribonucleic acid) was extracted from them. The yields of DNA was determined by the 2 methods. The both method of determination on DNA showed a similar tendency; the higher the exposed dose and the concentration of ethylene glycol, the better the yields.

摘要

在不同浓度乙二醇存在的情况下，对从大麦干种子胚中分离出的细胞核进行γ射线辐照后，从中提取大分子脱氧核糖核酸（DNA）。通过两种方法测定DNA的产量。两种DNA测定方法显示出相似的趋势；辐照剂量和乙二醇浓度越高，产量越好。