Salzberg S, Shurtz R, Feder J, Aboud M
Arch Virol. 1983;78(3-4):267-78. doi: 10.1007/BF01311321.
The effect of interferon (IFN) on the adsorption, penetration and subsequent binding of the incoming genome RNA of Moloney murine leukemia virus (MLV) to polyribosomes, was studied in NIH/3T3 mouse fibroblasts. Virus adsorption was assayed by determining reverse transcriptase activity in the inoculating virus stock and in the cell membrane fraction before and after 45 minutes of infection. Both measurements suggested that IFN had no effect on virus adsorption. Virus penetration was determined by measuring the amount of viral RNA in the cell cytoplasm at 45 minutes after infection. This amount was remarkably lower in IFN-treated than in untreated cells. This reduction was not due to inhibition of a possible induction of endogenous viral genetic information by the penetrating virions, but was proved to be a direct effect of IFN on virus penetration, which was related to the IFN-induced antiviral state. The effect of IFN on binding of parental genome RNA to polyribosomes was then investigated by analysing Crt hybridization kinetics of polyribosomal viral RNA at different time intervals after infection. While in untreated control cells maximal binding occurred at 3 hours postinfection, this maximal binding was observed in IFN-treated cells at 5 hours postinfection. The distribution of viral RNA molecules between sub-cytoplasmic fractions at 3 hours after infection was, in IFN-treated cells, significantly different from that observed in the untreated cells.
在NIH/3T3小鼠成纤维细胞中研究了干扰素（IFN）对莫洛尼鼠白血病病毒（MLV）传入基因组RNA吸附、穿透以及随后与多核糖体结合的影响。通过测定接种病毒原液以及感染45分钟前后细胞膜组分中的逆转录酶活性来检测病毒吸附情况。这两项测量结果均表明IFN对病毒吸附没有影响。通过测量感染后45分钟时细胞质中的病毒RNA量来确定病毒穿透情况。在经IFN处理的细胞中，该量显著低于未处理的细胞。这种减少并非由于穿透性病毒粒子对可能诱导的内源性病毒遗传信息的抑制，而是被证明是IFN对病毒穿透的直接作用，这与IFN诱导的抗病毒状态有关。然后通过分析感染后不同时间间隔多核糖体病毒RNA的Crt杂交动力学来研究IFN对亲本基因组RNA与多核糖体结合的影响。在未处理的对照细胞中，最大结合发生在感染后3小时，而在经IFN处理的细胞中，最大结合在感染后5小时观察到。在感染后3小时，经IFN处理的细胞中病毒RNA分子在细胞质亚组分之间的分布与未处理细胞中观察到的情况显著不同。
