Tomilin N V, Kuznetsov V G, Mashanskiĭ V F
Arkh Anat Gistol Embriol. 1983 Oct;85(10):67-74.
Distribution of calcium ions in the rat diaphragm muscle fibers has been studied electron histochemically using various fixation techniques and chemical treatment of the tissue. When potassium pyroantimonate in water solution is used after a short perfusate fixation with aldehydes, the reaction product granules are revealed in mitochondria, in the disk I, in the center of the disk A, more seldom the precipitate is found in the sarcoplasmic reticulum (SR) and in the T-system. The presence of calcium ions in the precipitate is proved by means of treatment the preparations with ethylenglycol- and ethylen-diamine-tetra-acetic acids. When contracture is resulted from potassium rhodanide administration, in mitochondria the reaction product granules decrease in their number, the precipitate disappears from the central part of the disk A, while the number of the granules increases in the SR terminal cisterns. The data obtained are compared with calcium ions distribution observed at the freezing-substitution method without an additional chemical fixation, as well as the histochemical fixations after Oschman method and at a usual fixation with OsO4. Certain similarity is revealed in distribution of the calcium pyroantimonate granules at aldehyde fixation and when the freezing-substitution method is used.
利用各种固定技术和对组织的化学处理，通过电子组织化学方法研究了大鼠膈肌肌纤维中钙离子的分布情况。在用醛类进行短时间灌注固定后，使用水溶液中的焦锑酸钾时，在线粒体、I带、A带中央可发现反应产物颗粒，在肌浆网（SR）和T系统中较少发现沉淀。通过用乙二醇和乙二胺四乙酸处理标本，证实了沉淀物中存在钙离子。当因注射硫氰酸钾导致挛缩时，线粒体中的反应产物颗粒数量减少，A带中央部分的沉淀消失，而SR终池中的颗粒数量增加。将所得数据与在无额外化学固定的冷冻置换法以及Oschman法后的组织化学固定和常规OsO4固定时观察到的钙离子分布进行了比较。在醛固定和使用冷冻置换法时，焦锑酸钙颗粒的分布显示出一定的相似性。
