荧光素钠对视网膜外植体培养中神经突生长的影响：一种视网膜毒性的体外模型。

Effect of sodium fluorescein on neurite outgrowth from the retinal explant culture: an in vitro model for retinal toxicity.

作者信息

Kato S, Madachi-Yamamoto S, Hayashi Y, Miki N, Negishi K

出版信息

Brain Res. 1983 Dec;313(1):143-7. doi: 10.1016/0165-3806(83)90211-0.

Abstract

Effect of sodium fluorescein was examined on neurite outgrowth from the retinal explants of chick embryos cultured with chicken gizzard extract, containing macromolecules which promote neurite outgrowth. A dose more than 0.5 mg/ml of fluorescein completely prevented the neurite outgrowth from the retinal explants. The minimum dose of toxic effect of fluorescein on the neurite outgrowth was about 0.2 mg/ml. The fluorescein also caused lysis of pre-existing neurites which had been fully extended by gizzard extract.

摘要

研究了荧光素钠对鸡胚视网膜外植体神经突生长的影响，这些外植体在含有促进神经突生长的大分子的鸡砂囊提取物中培养。荧光素剂量超过0.5毫克/毫升时，完全抑制了视网膜外植体的神经突生长。荧光素对神经突生长产生毒性作用的最小剂量约为0.2毫克/毫升。荧光素还导致已被砂囊提取物充分延伸的原有神经突发生溶解。