从大脑中提取的物质可刺激胎鼠视网膜外植体的神经突生长。

Extract from brain stimulates neurite outgrowth from fetal rat retinal explants.

作者信息

Turner J E, Barde Y A, Schwab M E, Thoenen H

出版信息

Brain Res. 1982 Dec;282(1):77-83. doi: 10.1016/0165-3806(82)90176-6.

Abstract

Explants from rat fetal retina were placed in culture and assayed for fiber outgrowth. In contrast to results obtained with lower vertebrates, nerve growth factor (NGF) does not seem to play a role in this system: NGF is not able to stimulate fiber outgrowth and antibodies to NGF do not block the spontaneously occurring fiber outgrowth. However, an extract prepared from pig brain is able to stimulate fiber outgrowth in a dose-dependent manner. It is suggested that such an extract can be used as a source of putative neurotrophic factors exhibiting in the mammalian central nervous system (CNS) an action similar to that of NGF in the peripheral nervous system (PNS) of mammals and in the CNS of lower vertebrates like fishes and amphibia.

摘要

将大鼠胎儿视网膜的外植体进行培养，并检测其纤维生长情况。与用低等脊椎动物获得的结果相反，神经生长因子（NGF）在该系统中似乎不起作用：NGF无法刺激纤维生长，而抗NGF抗体也不能阻断自发出现的纤维生长。然而，从猪脑中制备的提取物能够以剂量依赖的方式刺激纤维生长。有人提出，这种提取物可作为一种假定的神经营养因子的来源，该因子在哺乳动物中枢神经系统（CNS）中表现出与NGF在哺乳动物外周神经系统（PNS）以及鱼类和两栖类等低等脊椎动物的CNS中相似的作用。