Yang R, Fristensky B, Deutch A H, Huang R C, Tan Y H, Narang S A, Wu R
Gene. 1983 Nov;25(1):59-66. doi: 10.1016/0378-1119(83)90167-1.
Three cloned human DNA fragments obtained from a fibroblast genomic DNA were sequenced and identified as containing members of the well-known 300-bp Alu family of interspersed, middle-repetitive DNA sequences. One of these cloned DNA fragments, p16, also contains members of a new repetitive DNA family, which repeats several thousand times in the human genome. Each member of the new 528-bp family consists of eight tandem repeats of a 66-bp sequence. An AluI recognition site is present at the same location in each repeat, and a 25-bp sequence occurs twice (as a tandem repeat) in each of the eight repeats. There is no sequence homology between the new 528-bp family and the 300-bp Alu family, and the new family lacks the flanking 7- to 20-bp direct repeats as well as the dAMP-rich sequences characteristic of the 300-bp Alu family. Construction of a putative evolutionary tree indicates that six duplication events are needed to give rise to the eight tandemly repeated 66-bp units in the new 528-bp family.
从成纤维细胞基因组DNA中获得的三个克隆的人类DNA片段进行了测序，并被鉴定为包含散布的、中度重复的DNA序列中著名的300bp Alu家族的成员。这些克隆的DNA片段之一，p16，还包含一个新的重复DNA家族的成员，该家族在人类基因组中重复数千次。新的528bp家族的每个成员由一个66bp序列的八个串联重复组成。一个AluI识别位点存在于每个重复的相同位置，并且一个25bp的序列在八个重复中的每一个中出现两次（作为串联重复）。新的528bp家族与300bp Alu家族之间没有序列同源性，并且新家族缺乏侧翼7至20bp的直接重复以及300bp Alu家族特有的富含dAMP的序列。构建一个推测的进化树表明，需要六次复制事件才能产生新的528bp家族中的八个串联重复的66bp单元。
