The analysis of [14C]clofibric acid glucuronide and [14C]clofibric acid in plasma and urine using high-performance liquid chromatography.

A high-performance liquid chromatographic method has been developed for the quantitation of [14C]clofibric acid glucuronide and [14C]clofibric acid using conventional scintillation counting. The assay has a linear relationship between the added and observed ratios of clofibric acid glucuronide: clofibric acid in the range of 0.001-0.6 for plasma and 0.5-100 for urine, and is able to quantitate previously unmeasurable concentrations of clofibric acid glucuronide in plasma.