Barra J, Taburet A M, Jardel A, Fessi H, Puisieux F
Department of Pharmaceutical Technology, Université de Paris-Sud, Châtenay-Malabry, France.
J Chromatogr B Biomed Appl. 1994 Nov 4;661(1):178-82. doi: 10.1016/0378-4347(94)00336-x.
A rapid, sensitive, and selective HPLC assay was developed for the simultaneous determination of ethyl clofibrate and its major metabolite, clofibric acid, in plasma of humans and rats. The assay involves extraction of the compounds into chloroform-isoamyl alcohol (99:1, v/v) from plasma acidified with sulfuric acid. For human plasma, the overall recoveries of ethyl clofibrate and clofibric acid were 63 and 90%, respectively. The limits of detection of the assay for ethyl clofibrate and clofibric acid in human plasma were 1.1 and 1.5 micrograms/ml, respectively. Limits of quantitation of the assay for ethyl clofibrate and clofibric acid in human plasma were 3.6 and 4.9 micrograms/ml, respectively. The HPLC assay was used to monitor the plasma concentration-time profiles of ethyl clofibrate released from polylactic nanocapsules both in man and rat. The simultaneous determination of ethyl clofibrate and clofibric acid provided evidence that these colloidal systems are stable in human plasma whereas they are lysed in rat plasma.
建立了一种快速、灵敏且选择性高的高效液相色谱法（HPLC），用于同时测定人和大鼠血浆中氯贝丁酯及其主要代谢物氯贝酸的含量。该测定方法包括用硫酸酸化血浆后，将化合物萃取到氯仿 - 异戊醇（99:1，v/v）中。对于人血浆，氯贝丁酯和氯贝酸的总回收率分别为63%和90%。该测定方法对人血浆中氯贝丁酯和氯贝酸的检测限分别为1.1微克/毫升和1.5微克/毫升。该测定方法对人血浆中氯贝丁酯和氯贝酸的定量限分别为3.6微克/毫升和4.9微克/毫升。HPLC法用于监测聚乳酸纳米胶囊释放的氯贝丁酯在人和大鼠体内的血浆浓度 - 时间曲线。同时测定氯贝丁酯和氯贝酸表明，这些胶体系统在人血浆中稳定，而在大鼠血浆中会被裂解。
