Determination of N-mononitrosopiperazine and N,N'-dinitrosopiperazine in human urine, gastric juice and blood.

A rapid, selective and sensitive method for the determination of N-mononitrosopiperazine and N,N'-dinitrosopiperazine in human urine, gastric juice and blood has been developed using gas-liquid chromatography with thermal energy analysis. The compounds were isolated by extraction and Extrelut with dichloromethane or chloroform. The detection limits were between 0.2 and 2 ng/ml.