Cheng C C, Sege K, Alton A K, Bennett D, Artzt K
J Immunogenet. 1983 Dec;10(6):465-85. doi: 10.1111/j.1744-313x.1983.tb01033.x.
In attempts to identify cell surface molecules specified by lethal genes in the T/t-complex, we prepared a rabbit antiserum that has cytotoxic activity against testicular cells from males heterozygous for t12, but not against wild type cells. However, anti-t12 serum immunoprecipitates the same major component, a glycoprotein of mol. wt. 87,000 daltons, from galactose-labelled C3H. +/t12 testicular cell lysate and from congenic C3H. +/+ lysate, although the gp87 molecule precipitated from +/t12 cells appears to be more highly galactosylated than the +/+ form. The antigen is heavily glycosylated in both genotypes, since when testicular cells are treated with tunicamycin before immunoprecipitation, a protein of 40,000-42,000 daltons is obtained. Gp87 is also present on pre-implantation embryos, and on teratocarcinoma cells, but is barely detectable on any adult somatic cells examined. Its expression is developmentally regulated during pre-implantation stages, but the temporal pattern of its expression appears to be different between wild type and t12 embryos. Thus, we believe we have identified a molecule that may play a role in the differentiation of testicular cells and pre-implantation embryos, and that is either specified by genes in the t12 haplotype, or responsive in some way to the effects of t12.
为了鉴定T/t复合体内致死基因所特有的细胞表面分子，我们制备了一种兔抗血清，它对携带t12的杂合雄性小鼠的睾丸细胞具有细胞毒性活性，但对野生型细胞没有细胞毒性。然而，抗t12血清从半乳糖标记的C3H. +/t12睾丸细胞裂解物和同基因的C3H. +/+裂解物中免疫沉淀出相同的主要成分，一种分子量为87,000道尔顿的糖蛋白，尽管从+/t12细胞中沉淀出的gp87分子似乎比+/+形式的糖基化程度更高。在两种基因型中，该抗原都高度糖基化，因为在免疫沉淀前用衣霉素处理睾丸细胞时，可得到一种40,000 - 42,000道尔顿的蛋白质。Gp87也存在于植入前胚胎和畸胎癌细胞上，但在任何检测的成年体细胞中几乎检测不到。它的表达在植入前阶段受到发育调控，但其表达的时间模式在野生型和t12胚胎之间似乎有所不同。因此，我们认为我们已经鉴定出一种可能在睾丸细胞和植入前胚胎的分化中起作用的分子，它要么由t12单倍型中的基因所特化，要么以某种方式对t12的作用有反应。
