Spielmann H, Erickson R P
J Embryol Exp Morphol. 1983 Dec;78:43-51.
The recently improved firefly luciferase assay was used to determine ATP, ADP or AMP in single preimplantation mouse embryos from crosses yielding lethal t12/t12 embryos. Normal values of the three adenylate ribonucleotides were found in freshly collected 2-cell and 4-cell embryos and during in vitro culture to the blastocyst stage. A decrease in adenylate ribonucleotide content was seen in putative t12/t12 embryos only when they were degenerating.
最近改进的萤火虫荧光素酶测定法被用于测定来自产生致死性t12/t12胚胎的杂交实验的单个植入前小鼠胚胎中的ATP、ADP或AMP。在新鲜收集的2细胞和4细胞胚胎以及体外培养至囊胚阶段的过程中，发现三种腺苷酸核糖核苷酸的正常值。只有在假定的t12/t12胚胎退化时，才会观察到腺苷酸核糖核苷酸含量的下降。
