Goda T, Hosoya N, Moriuchi S
J Nutr Sci Vitaminol (Tokyo). 1983 Oct;29(5):571-8. doi: 10.3177/jnsv.29.571.
The changes in the activity and content of sucrase-isomaltase complex (S-I) in the intestinal mucosa were studied during the development of diabetes induced by streptozotocin in rats. On days 0, 1, 3, 5, and 10 after an intraperitoneal injection of streptozotocin (70 mg/kg), the enzyme activity and the enzyme content were observed in the jejunum and ileum. Sucrase and isomaltase activities markedly increased from the 3rd day both in the jejunum and ileum, and kept increasing till the 10th day especially in the ileum. The enzyme content of S-I also increased in parallel with its activity during the development of diabetes. However, in the early stage of diabetes, sucrase activity per microgram of S-I content increased both in the jejunum and ileum. Isomaltase activity per microgram of S-I content increased temporarily in the ileum. These results suggest that the increase of disaccharidase activities in the early stage of diabetes induced by streptozotocin is not only due to the increase of the enzyme content, but also due to the change of the enzyme catalytic property.
在链脲佐菌素诱导的大鼠糖尿病发展过程中，研究了肠黏膜中蔗糖酶 - 异麦芽糖酶复合物（S - I）的活性和含量变化。在腹腔注射链脲佐菌素（70 mg/kg）后的第0、1、3、5和10天，观察空肠和回肠中的酶活性和酶含量。蔗糖酶和异麦芽糖酶活性从第3天开始在空肠和回肠中均显著增加，并持续增加直至第10天，尤其是在回肠中。在糖尿病发展过程中，S - I的酶含量也与其活性平行增加。然而，在糖尿病早期，每微克S - I含量的蔗糖酶活性在空肠和回肠中均增加。每微克S - I含量的异麦芽糖酶活性在回肠中暂时增加。这些结果表明，链脲佐菌素诱导的糖尿病早期双糖酶活性的增加不仅是由于酶含量的增加，还由于酶催化特性的改变。
