Zager R A, Venkatachalam M A
Kidney Int. 1983 Nov;24(5):620-5. doi: 10.1038/ki.1983.202.
The purpose of this study was to determine whether amino acid hyperalimentation sensitizes the kidney to ischemic renal injury. Sprague-Dawley rats were infused with a control infusate or control infusate plus 15 essential and nonessential amino acids (FreAmine III, 2 mg of amino acids/kg/min). After 1 hr, bilateral renal ischemia was induced (15 min pedicle cross clamp). GFR, urine flow, and renal blood flow (RBF) were measured before and for 0 to 2 hr postischemia. None of these parameters were statistically different between the two groups of rats prior to renal ischemia. However, the decrease in GFR following renal ischemia was twice as great in the amino acid versus the nonamino acid treatment group (decreases 73 +/- 4% vs. decreases 36 +/- 4%, respectively; P less than 0.001). The postischemia urine flow rate was also significantly less (P less than 0.01) with amino acid treatment. RBF did not differ between the two groups. Renal histology confirmed greater tubular injury with amino acid treatment (P less than 0.001). In conclusion, infusion of therapeutic doses of amino acids can exacerbate early functional and histologic parameters of ischemic renal injury and thus, may sensitize the kidney to ischemic acute renal failure.
本研究的目的是确定氨基酸高营养疗法是否会使肾脏对缺血性肾损伤更敏感。给Sprague-Dawley大鼠输注对照输注液或对照输注液加15种必需和非必需氨基酸（复方氨基酸注射液III，2mg氨基酸/（kg·min））。1小时后，诱导双侧肾缺血（肾蒂交叉夹闭15分钟）。在缺血前以及缺血后0至2小时测量肾小球滤过率（GFR）、尿流量和肾血流量（RBF）。在肾缺血前，两组大鼠的这些参数均无统计学差异。然而，肾缺血后，氨基酸治疗组的GFR下降幅度是非氨基酸治疗组的两倍（分别下降73±4%和36±4%；P<0.001）。氨基酸治疗后的缺血后尿流率也显著降低（P<0.01）。两组之间的RBF没有差异。肾脏组织学检查证实氨基酸治疗导致更严重的肾小管损伤（P<0.001）。总之，输注治疗剂量的氨基酸会加重缺血性肾损伤的早期功能和组织学参数，因此，可能会使肾脏对缺血性急性肾衰竭更敏感。
