[3H]γ-羟基丁酸在大鼠小脑和脊髓培养神经元上的结合位点

Binding sites for [3H]gamma-hydroxybutyrate on cultured neurones of rat cerebellum and spinal cord.

作者信息

Hösli E, Hösli L

出版信息

Neurosci Lett. 1983 Dec 2;42(2):145-8. doi: 10.1016/0304-3940(83)90397-x.

Abstract

By means of light microscopic autoradiography, binding sites for the GABA catabolite [3H]gamma-hydroxybutyrate (GHB) were observed on cultured cerebellar and spinal neurones but not on glial cells. [3H]GHB was bound to similar types of neurones as [3H]GABA. However, the number of neurones labelled by [3H]GHB was considerably smaller than that by [3H]GABA, and the intensity of labelling by [3H]GHB was usually weaker. It is suggested that GHB might interact with neurotransmission mediated by GABA or play a role as neuromodulator or neurotransmitter in the mammalian central nervous system.

摘要

通过光学显微镜放射自显影术，在培养的小脑和脊髓神经元上观察到γ-氨基丁酸（GABA）分解代谢物[3H]γ-羟基丁酸（GHB）的结合位点，而在胶质细胞上未观察到。[3H]GHB与[3H]GABA结合于相似类型的神经元。然而，被[3H]GHB标记的神经元数量比被[3H]GABA标记的神经元数量少得多，并且[3H]GHB的标记强度通常较弱。这表明GHB可能与GABA介导的神经传递相互作用，或在哺乳动物中枢神经系统中作为神经调质或神经递质发挥作用。