Wettre S, Arnqvist H, von Schenck H
Scand J Clin Lab Invest. 1983 Dec;43(8):739-45. doi: 10.1080/00365518309168858.
Incubation of erythrocytes from diabetic patients and non-diabetic persons in 0.15 mol/l saline at 37 degrees C was followed by a decrease in ion-exchange microcolumn determined HbA1 only during the first 4 h. The fraction removed was denoted as labile HbA1 and the remaining fraction as stable HbA1. Incubation in saline overnight at 20 degrees C did not completely remove the labile fraction. Incubation in saline overnight at 4 degrees C yielded only a slight decrease in labile HbA1. Incubation in saline containing various glucose concentrations (up to 100 mmol/l) produced increasing amounts of HbA1 during the first 3-4 h after which an equilibrium was reached. In both normal and diabetic erythrocytes incubated for 3-4 h we found a linear relationship between changes in labile HbA1 and glucose concentration. The degree of increase in labile HbA1 was the same in normals and diabetics and not dependent on pre-existing normal or moderately increased stable HbA1. In the blood from non-diabetics (n = 30) labile HbA1 was 0.5 +/- 0.3% (mean +/- SD) and in diabetics (n = 80) 1.6 +/- 0.8%. The correlation between labile HbA1 and simultaneously determined blood glucose was r = 0.72.
将糖尿病患者和非糖尿病患者的红细胞在37℃下于0.15mol/l盐水中孵育，仅在最初4小时内离子交换微柱测定的糖化血红蛋白（HbA1）会下降。去除的部分称为不稳定HbA1，其余部分称为稳定HbA1。在20℃盐水中过夜孵育不能完全去除不稳定部分。在4℃盐水中过夜孵育只会使不稳定HbA1略有下降。在含有不同葡萄糖浓度（高达100mmol/l）的盐水中孵育，在最初3 - 4小时内会产生越来越多的HbA1，之后达到平衡。在孵育3 - 4小时的正常和糖尿病红细胞中，我们发现不稳定HbA1的变化与葡萄糖浓度之间呈线性关系。不稳定HbA1的增加程度在正常人和糖尿病患者中相同，且不依赖于预先存在的正常或中度升高的稳定HbA1。在非糖尿病患者（n = 30）的血液中，不稳定HbA1为0.5±0.3%（平均值±标准差），在糖尿病患者（n = 80）中为1.6±0.8%。不稳定HbA1与同时测定的血糖之间的相关性为r = 0.72。
