Gibbs R S, Blanco J D, St Clair P J, Castaneda Y S
Sex Transm Dis. 1983 Oct-Dec;10(4 Suppl):303-6.
Amniotic fluid was collected via a transcervical intrauterine catheter from patients with clinical evidence of intrauterine infection and from uninfected comparison patients. The amniotic fluid was cultured for Mycoplasma hominis and Ureaplasma urealyticum, as well as for aerobic and anaerobic bacteria. Two series of patients are reported. In the first, there were 52 patients with intraamniotic infection and 52 matched controls. Eighteen patients (35%) with intraamniotic infection and four control patients (8%) had M. hominis in the amniotic fluid (P less than .001). Twenty-six patients (50%) with intraamniotic infection and 26 control patients (50%) had U. urealyticum in the amniotic fluid. In the second series, samples were collected consecutively. M. hominis was isolated from 32% (19/60) of specimens from patients with signs of infection and from 14% (8/56) of afebrile comparison patients (P less than .02). U. urealyticum was recovered from 43% and 39% of these fluids, respectively; the difference was not significant. When M. hominis was found in amniotic fluids of patients with signs of infection, the amniotic fluid usually contained greater than or equal to 10(2) cfu of a high virulent bacterial isolate per milliliter. Qualitative cultures showed that M. hominis in the amniotic fluid is associated with clinical intrauterine infection.
通过经宫颈子宫内导管从有宫内感染临床证据的患者以及未感染的对照患者中采集羊水。对羊水进行人型支原体、解脲脲原体以及需氧菌和厌氧菌培养。报告了两个系列的患者。在第一个系列中，有52例羊膜内感染患者和52例匹配的对照。18例（35%）羊膜内感染患者和4例对照患者（8%）的羊水中有人型支原体（P<0.001）。26例（50%）羊膜内感染患者和26例对照患者（50%）的羊水中有解脲脲原体。在第二个系列中，样本是连续采集的。从有感染体征患者的32%（19/60）的标本以及无发热对照患者的14%（8/56）的标本中分离出人型支原体（P<0.02）。解脲脲原体分别从这些羊水的43%和39%中检出；差异无统计学意义。当在有感染体征患者的羊水中发现人型支原体时，羊水通常每毫升含有≥10(2) cfu的高毒力细菌分离株。定性培养显示羊水中的人型支原体与临床宫内感染有关。
