Antonov S, Malchevski M
Vet Med Nauki. 1983;20(9):3-11.
Studied were the total activity and the type of isoenzymes of the alkaline phosphatase in blood serum of cattle, sheep, and swine at various ages and different physiologic status. With all three species the enzyme activity showed explicit age dynamic. In month-old calves the activity of the serum phosphatase was 82.8 IU, on an average, and in adult cows--32.2 IU. In month-old lambs the enzyme activity was 310.6 IU, on an average, while in adult sheep it was 62.0 IU. In young pigs it ranged up to 105.0 IU, and in adult swine--up to 32.4 IU. No statistically significant differences in the enzyme activity was established between dry and lactating cows and ewes as well as between rams, boars, and sows. The study on the isoenzymes of the alkaline phosphatase (thermostability, specific chemoinhibitors, and electrophoresis in agar gel) revealed that in month-old calves there was only a bone isoenzyme in the blood serum; in calves that were more advanced in age there appeared also the liver and the intestinal isoenzymes, while in adult cows the intestinal or the liver enzyme of alkaline phosphatase was present only. The blood serum of lambs up to the sixth month of age contained the bone and the intestinal isoenzymes, and that of adult sheep--the liver and the intestinal ones. In pregnant sheep there were even low amounts of the placental isoenzyme of alkaline phosphatase. In pigs up to the sixth month of age the serum phosphatase was shown to be of bone origin, while in the adult swine--chiefly of liver origin.
研究了不同年龄和不同生理状态的牛、羊和猪血清中碱性磷酸酶的总活性及同工酶类型。在这三种动物中，酶活性均呈现出明显的年龄动态变化。1月龄犊牛血清磷酸酶活性平均为82.8 IU，成年母牛为32.2 IU。1月龄羔羊酶活性平均为310.6 IU，成年羊为62.0 IU。幼猪酶活性最高可达105.0 IU，成年猪最高可达32.4 IU。在干奶期母牛和泌乳期母牛以及母羊之间，以及在公羊、公猪和母猪之间，酶活性未发现有统计学上的显著差异。对碱性磷酸酶同工酶（热稳定性、特异性化学抑制剂以及琼脂凝胶电泳）的研究表明，1月龄犊牛血清中只有骨同工酶；年龄稍大的犊牛血清中还出现了肝同工酶和肠同工酶，而成年母牛血清中仅存在肠碱性磷酸酶或肝碱性磷酸酶。6月龄以下羔羊的血清中含有骨同工酶和肠同工酶，成年羊血清中含有肝同工酶和肠同工酶。怀孕母羊血清中甚至含有少量碱性磷酸酶的胎盘同工酶。6月龄以下仔猪血清磷酸酶显示为骨源性，而成年猪血清磷酸酶主要为肝源性。
