[Protein fractions in diluted bull seminal fluid with various biological indices after thermal treatment].

Studied were electrophoretically the protein fractions of bull semen after heat treatment of the GH22L extender. Studied were also the changes in the protein fractions of diluted semen of various biologic indices. It was found that after heat treatment of the extender at 70 degrees C for 60 min. most stable proved the fractions that were at the front and the start. Negligible changes underwent the proteins of the 3rd and 4th fraction. The changes in the percent distribution of the protein fractions of diluted qualitative and unqualitative bull semen at 1+2 degree with thermically untreated GH22L extender were best manifested with the semen of lower quality. Weaker were the changes taking place with dilution of qualitative and unqualitative semen at 1+6 degree. These changes were observed also with dilution at 1+2 with thermically treated extender (70 degrees C for 60 min.). Dilution of qualitative semen with thermically treated (at 1+6) extender most strongly changed the proteins of the 1st, 2nd, and 3rd fraction. The proteins of the start showed high stability. Nonsignificant were the changes with unqualitative semen with which all fractions showed great heat stability.