Kichev G, Al-Hanak H, Nauk V
Vet Med Nauki. 1981;18(6):52-7.
Electrophoresis on celogel and staining the bands with Schiff's agent were used to study the sugar fractions in the diluents Nagase Niva, Triladil, Leciphos and GH22L (after Kichev) as well as in bull semen diluted to a various extent with the same media. Using combined staining--sugars/proteins--it was found that all sugar fractions in both media and diluted semen were bound with proteins. These biocomplexes were found to lower their electrophoretic mobility with the rising values of dilution. Discussed is the possibility to use the conformation of sugar complexes in the working out of new cryoprotective media.
采用在赛洛凝胶上进行电泳并用席夫试剂对条带进行染色的方法，研究了长濑尼瓦、三利多、卵磷脂和GH22L（基切夫之后）稀释液中的糖组分，以及用相同介质不同程度稀释的公牛精液中的糖组分。通过糖/蛋白质联合染色发现，两种介质和稀释精液中的所有糖组分均与蛋白质结合。随着稀释度值的升高，发现这些生物复合物的电泳迁移率降低。讨论了在研制新型冷冻保护介质时利用糖复合物构象的可能性。
