Kleinman D, Sharon Y, Sarov I, Insler V
Arch Gynecol. 1983;234(2):103-12. doi: 10.1007/BF00207682.
The present study describes a simple method for culturing human endometrium as separate epithelial and stromal components. Fifty-two samples of normal human endometrium have been initiated in tissue culture: endometrium from both the proliferative and luteal phases of the menstrual cycle showed satisfactory growth in vitro with a success rate of about 94%. Epithelial cultures remained viable for 60 days, while from stromal cells it was possible to establish cell lines. Both cell types possessed estrogen receptors. Epithelial cells showed no clear estrogen or progesterone response. Our observations suggest that this simple method for culturing human endometrium may serve as a tool in further investigations.
本研究描述了一种将人子宫内膜作为独立的上皮和基质成分进行培养的简单方法。52份正常人子宫内膜样本已开始进行组织培养：月经周期增殖期和黄体期的子宫内膜在体外均显示出良好的生长，成功率约为94%。上皮培养物可存活60天，而从基质细胞中有可能建立细胞系。两种细胞类型均具有雌激素受体。上皮细胞未显示出明显的雌激素或孕激素反应。我们的观察结果表明，这种简单的人子宫内膜培养方法可能成为进一步研究的一种工具。
