来自正常人类子宫内膜的有限细胞系的建立及形态学特征分析

The establishment and morphologic characterization of finite cell lines from normal human endometrium.

作者信息

Trent J M, Davis J R, Payne C M

出版信息

Am J Obstet Gynecol. 1980 Feb 1;136(3):352-62. doi: 10.1016/0002-9378(80)90862-5.

Abstract

Twenty-nine samples of normal human endometrium have been initiated in tissue culture. Twenty cultures (69%) demonstrated sufficient growth in vitro to allow establishment of finite cell lines. Morphologic characterization by means of conventional light microscopy as well as scanning and transmission electron microscopy was performed. Differences between stromal and epithelial cell lines indicated several similarities with in vivo observations. Ultrastructural studies of stromal cell lines revealed cells virtually devoid of microvilli and exhibiting poorly specialized cell junctions. Epithelial cell lines were characterized by numerous microvilli and highly specialized "junctional complexes" typical of glandular epithelium. Cytogenetic analysis utilizing a variety of banding techniques revealed no karyotypic alterations through several subpassages. Our observations suggest that simple explant tissue culture of human endometrium can be manipulated in order to achieve separation of stromal from epithelial elements, allowing critical examination of each.

摘要

已对29份正常人子宫内膜样本进行了组织培养。20份培养物（69%）在体外显示出足够的生长，从而能够建立有限细胞系。通过传统光学显微镜以及扫描和透射电子显微镜进行了形态学特征分析。基质细胞系和上皮细胞系之间的差异表明与体内观察结果有若干相似之处。基质细胞系的超微结构研究显示细胞几乎没有微绒毛，且细胞连接特化程度低。上皮细胞系的特征是有大量微绒毛以及腺上皮典型的高度特化的“连接复合体”。利用多种显带技术进行的细胞遗传学分析显示，经过数次传代培养后染色体核型没有改变。我们的观察结果表明，可以对人子宫内膜的简单外植体组织培养进行操作，以实现基质成分与上皮成分的分离，从而对每种成分进行严格检查。