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Chymotryptic and tryptic peptides of fragment alpha 1-CB3 from bovine corneal collagen. Pinpointing the sites of hexose attachment.

作者信息

Panjwani N A, Harding J J

出版信息

Biochem J. 1978 Jun 1;171(3):697-703. doi: 10.1042/bj1710697.

Abstract

Tryptic peptides of citraconylated fragment alpha1-CB3 and chymotryptic peptides of fragment alpha1-CB3 of bovine corneal collagen were prepared, isolated and characterized. Their amino acid compositions were consistent with the amino acid sequence of fragment alpha1-CB3 from calf skin collagen. Two glycoside sites were identified in bovine corneal fragment alpha1-CB3, one of them being the first located in the overlap region of collagen. The results are related to the uniformly narrow collagen fibres found in cornea and essential for its transparency.

摘要

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引用本文的文献

本文引用的文献

1
Evidence for a non-helical region at the carboxyl terminus of the collagen molecule.
FEBS Lett. 1971 Feb 19;13(2):101-104. doi: 10.1016/0014-5793(71)80209-0.
2
Variations in the carbohydrate content of human and bovine polymeric collagens from various tissues.
Biochim Biophys Acta. 1969 May 6;177(3):682-5. doi: 10.1016/0304-4165(69)90345-6.
3
Reversible blocking of amino groups with citraconic anhydride.
Biochem J. 1968 Sep;109(2):312-4. doi: 10.1042/bj1090312.
6
The covalent structure of collagen: amino acid sequence of 1-CB3 from calf skin collagen.
FEBS Lett. 1972 Oct 1;26(1):74-6. doi: 10.1016/0014-5793(72)80545-3.
7
Hydroxylysine glycosides in the collagen of normal and scarred rabbit corneas.
Biochem Biophys Res Commun. 1974 Sep 9;60(1):288-94. doi: 10.1016/0006-291x(74)90203-4.
9
The distribution of carbohydrate groups in rat skin collagen.
J Biol Chem. 1973 Jul 25;248(14):5106-13.

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