甘氨酸N-酰基转移酶活性的放射化学测定。大鼠和家兔体内该酶的一些特性。
A radiochemical assay for glycine N-acyltransferase activity. Some properties of the enzyme in rat and rabbit.
作者信息
James M O, Bend J R
出版信息
Biochem J. 1978 May 15;172(2):285-91. doi: 10.1042/bj1720285.
Abstract
We have developed a sensitive radiochemical assay of glycine N-acyltransferase activity, using phenylacetyl-CoA as the acyl donor and glycine as the acceptor. This assay measures formation of the product, phenylacetylglycine, instead of disappearance of the substrate, phenylacetyl-CoA, as did earlier assays. The subcellular location and some properties of the conjugating activity were determined in liver and kidney of the rabbit and the rat. Rabbit lung and intestine were also tested for activity.
摘要
我们开发了一种灵敏的甘氨酸N-酰基转移酶活性放射化学测定法，以苯乙酰辅酶A作为酰基供体，甘氨酸作为受体。该测定法测量产物苯乙酰甘氨酸的形成，而不像早期的测定法那样测量底物苯乙酰辅酶A的消失。在兔和大鼠的肝脏和肾脏中确定了结合活性的亚细胞定位和一些特性。还检测了兔肺和肠的活性。
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