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甘氨酸N-酰基转移酶活性的放射化学测定。大鼠和家兔体内该酶的一些特性。

A radiochemical assay for glycine N-acyltransferase activity. Some properties of the enzyme in rat and rabbit.

作者信息

James M O, Bend J R

出版信息

Biochem J. 1978 May 15;172(2):285-91. doi: 10.1042/bj1720285.

DOI:10.1042/bj1720285
PMID:666745
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1185694/
Abstract

We have developed a sensitive radiochemical assay of glycine N-acyltransferase activity, using phenylacetyl-CoA as the acyl donor and glycine as the acceptor. This assay measures formation of the product, phenylacetylglycine, instead of disappearance of the substrate, phenylacetyl-CoA, as did earlier assays. The subcellular location and some properties of the conjugating activity were determined in liver and kidney of the rabbit and the rat. Rabbit lung and intestine were also tested for activity.

摘要

我们开发了一种灵敏的甘氨酸N-酰基转移酶活性放射化学测定法，以苯乙酰辅酶A作为酰基供体，甘氨酸作为受体。该测定法测量产物苯乙酰甘氨酸的形成，而不像早期的测定法那样测量底物苯乙酰辅酶A的消失。在兔和大鼠的肝脏和肾脏中确定了结合活性的亚细胞定位和一些特性。还检测了兔肺和肠的活性。

相似文献

1
A radiochemical assay for glycine N-acyltransferase activity. Some properties of the enzyme in rat and rabbit.甘氨酸N-酰基转移酶活性的放射化学测定。大鼠和家兔体内该酶的一些特性。
Biochem J. 1978 May 15;172(2):285-91. doi: 10.1042/bj1720285.
2
Perinatal development of, and effect of chemical pretreatment on, glycine N-acyltransferase activities in liver and kidney of rabbit and rat.兔和大鼠肝脏及肾脏中甘氨酸N-酰基转移酶活性的围产期发育及化学预处理的影响
Biochem J. 1978 May 15;172(2):293-9. doi: 10.1042/bj1720293.
3
Benzoyl-coenzyme A:glycine N-acyltransferase and phenylacetyl-coenzyme A:glycine N-acyltransferase from bovine liver mitochondria. Purification and characterization.来自牛肝线粒体的苯甲酰辅酶A：甘氨酸N-酰基转移酶和苯乙酰辅酶A：甘氨酸N-酰基转移酶。纯化与特性鉴定。
J Biol Chem. 1979 Aug 10;254(15):7230-7.
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Identification of bile acid-CoA: amino acid N-acyltransferase in rat kidney.大鼠肾脏中胆汁酸辅酶A：氨基酸N-酰基转移酶的鉴定
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Isolation and characterization of mitochondrial acyl-CoA: glycine N-acyltransferases from kidney.肾脏线粒体酰基辅酶A：甘氨酸N-酰基转移酶的分离与鉴定
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Identification of separate acyl- CoA:glycine and acyl-CoA:L-glutamine N-acyltransferase activities in mitochondrial fractions from liver of rhesus monkey and man.恒河猴和人类肝脏线粒体组分中酰基辅酶A：甘氨酸和酰基辅酶A：L-谷氨酰胺N-酰基转移酶活性的鉴定。
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Photoaffinity labeling of acyl-coenzyme A:glycine N-acyltransferase with p-azidobenzoyl-coenzyme A.用对叠氮苯甲酰辅酶A对酰基辅酶A：甘氨酸N-酰基转移酶进行光亲和标记。
Biochemistry. 1977 Jun 14;16(12):2581-5. doi: 10.1021/bi00631a001.

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Stereoselective taurine conjugation of (R)-benoxaprofen enantiomer in rats: in vivo and in vitro studies using rat hepatic mitochondria and microsomes.大鼠体内（R）-苯恶洛芬对映体的立体选择性牛磺酸结合：使用大鼠肝线粒体和微粒体的体内和体外研究
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The aromatization of cyclohexanecarboxyl-CoA to hippuric acid by guinea pig liver mitochondria: submitochondrial localization.豚鼠肝脏线粒体将环己烷甲酰辅酶A芳构化为马尿酸：亚线粒体定位
Mol Cell Biochem. 1985 Jul;67(2):181-8. doi: 10.1007/BF02370177.
4
Perinatal development of, and effect of chemical pretreatment on, glycine N-acyltransferase activities in liver and kidney of rabbit and rat.兔和大鼠肝脏及肾脏中甘氨酸N-酰基转移酶活性的围产期发育及化学预处理的影响
Biochem J. 1978 May 15;172(2):293-9. doi: 10.1042/bj1720293.

本文引用的文献

1
Studies on 3-Indoleacetic Acid Metabolism. IV. Conjugation with Aspartic Acid and Ammonia as Processes in the Metabolism of Carboxylic Acids.3-吲哚乙酸代谢的研究。IV. 与天冬氨酸和氨结合作为羧酸代谢过程
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Computer programmes for processing enzyme kinetic data.用于处理酶动力学数据的计算机程序。
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Synthesis of phenylacetylglutamine by human tissue.人体组织合成苯乙酰谷氨酰胺。
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7
Tissue fractionation studies. 6. Intracellular distribution patterns of enzymes in rat-liver tissue.组织分级分离研究。6. 大鼠肝脏组织中酶的细胞内分布模式。
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Glycine N-acylase: purification and properties.甘氨酸N-酰基转移酶：纯化与性质
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9
The synthesis of taurocholic and glycocholic acids by preparations of human liver. I. Distribution of activity between subcellular fractions.人肝制剂合成牛磺胆酸和甘氨胆酸。I. 亚细胞组分间的活性分布
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10
Drug biotransformation interactions in man. II. A pharmacokinetic study of the simultaneous conjugation of benzoic and salicylic acids with glycine.人体中的药物生物转化相互作用。II. 苯甲酸和水杨酸与甘氨酸同时结合的药代动力学研究。
J Pharm Sci. 1969 Mar;58(3):321-6. doi: 10.1002/jps.2600580307.