极少量血清样本中甲氧苄啶的放射酶分析法。
Radioenzymatic assay for trimethoprim in very small serum samples.
作者信息
Yogev R, Melick C, Tan-Pong L
出版信息
J Clin Microbiol. 1985 Feb;21(2):249-50. doi: 10.1128/jcm.21.2.249-250.1985.
Abstract
A modification of the methotrexate radioassay kit (supplied by New England Enzyme Center) enabled determination of trimethoprim levels in 5-microliter serum samples. An excellent correlation between this assay and high-pressure liquid chromatography assay was found. These preliminary results suggest that with this method rapid determination of trimethoprim levels in very small samples (5 to 10 microliters) can be achieved.
摘要
对甲氨蝶呤放射分析试剂盒（由新英格兰酶中心提供）进行改良后，能够测定5微升血清样本中的甲氧苄啶水平。结果发现该分析方法与高压液相色谱分析方法之间具有良好的相关性。这些初步结果表明，采用这种方法能够快速测定极少量样本（5至10微升）中的甲氧苄啶水平。
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本文引用的文献
1
High-performance liquid chromatographic quantitation of trimethoprim, sulfamethoxazole, and N4-acetylsulfamethoxazole in body fluids.体液中甲氧苄啶、磺胺甲恶唑和N4-乙酰磺胺甲恶唑的高效液相色谱定量分析
J Chromatogr. 1983 Dec 9;278(2):337-45. doi: 10.1016/s0378-4347(00)84793-3.
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Rapid assay for determination of trimethoprim and sulfamethoxazole levels in serum by spectrofluorometry.用荧光分光光度法快速测定血清中甲氧苄啶和磺胺甲恶唑水平的方法
Antimicrob Agents Chemother. 1979 Nov;16(5):579-83. doi: 10.1128/AAC.16.5.579.
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Management of P. carinii pneumonia.卡氏肺孢子虫肺炎的管理
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