Barden J A
Anal Biochem. 1983 Nov;135(1):52-7. doi: 10.1016/0003-2697(83)90729-7.
A high-performance size exclusion liquid chromatographic system has been used to separate proteins with different shapes solely on the basis of their molecular weights. After the effects of ionic and hydrophobic interactions with the stationary phase have been overcome, protein elution is normally governed by their effective size in solution. Conditions are described under which proteins, with isoelectric points within the normal operating pH range of the columns, are eluted independent of their Stokes' radii. Even fibrous proteins with axial ratios of 50 elute according to their known molecular weights over the range 2000-2,000,000.
一种高效尺寸排阻液相色谱系统已被用于仅根据分子量来分离不同形状的蛋白质。在克服了与固定相的离子和疏水相互作用的影响之后，蛋白质的洗脱通常由其在溶液中的有效尺寸决定。本文描述了在何种条件下，等电点在柱子正常操作pH范围内的蛋白质能够独立于其斯托克斯半径被洗脱。即使是轴比为50的纤维状蛋白质，在2000至2000000的分子量范围内，也能根据其已知分子量被洗脱。
