对硝基苯基α-D-吡喃葡萄糖苷，一种用于葡聚糖蔗糖酶的新底物。

p-Nitrophenyl alpha-D-glucopyranoside, a new substrate for glucansucrases.

作者信息

Binder T P, Robyt J F

出版信息

Carbohydr Res. 1983 Dec 23;124(2):287-99. doi: 10.1016/0008-6215(83)88464-x.

Abstract

p-Nitrophenyl alpha-D-glucopyranoside has been shown to be a substrate for the glucansucrases of various strains of Leuconostoc mesenteroides and Streptococcus mutans. The products from a digest of p-nitrophenyl alpha-D-glucopyranoside with L. mesenteroides B-512F dextransucrase were found to include dextran, a series of p-nitrophenyl isomaltodextrin glycosides, and p-nitrophenyl nigeroside. The kinetics of the reaction were non-Michaelis-Menten, possibly because p-nitrophenyl alpha-D-glucopyranoside has a dual role in the reaction as both a D-glucosyl donor and acceptor.

摘要

对硝基苯基α-D-吡喃葡萄糖苷已被证明是多种肠系膜明串珠菌和变形链球菌菌株的葡聚糖蔗糖酶的底物。用肠系膜明串珠菌B-512F葡聚糖蔗糖酶消化对硝基苯基α-D-吡喃葡萄糖苷的产物包括葡聚糖、一系列对硝基苯基异麦芽糖糊精糖苷和对硝基苯基黑曲霉糖苷。该反应的动力学不符合米氏方程，可能是因为对硝基苯基α-D-吡喃葡萄糖苷在反应中作为D-葡萄糖基供体和受体具有双重作用。