Bertrand Anne, Morel Sandrine, Lefoulon François, Rolland Yves, Monsan Pierre, Remaud-Simeon Magali
Laboratoire Biotechnologie-Bioprocédés UMR CNRS 5504, UMR INRA 792, INSA DGBA, 135 avenue de Rangueil, 31077 Toulouse Cedex 04, France.
Carbohydr Res. 2006 May 22;341(7):855-63. doi: 10.1016/j.carres.2006.02.008. Epub 2006 Mar 10.
The enzymatic glucosylation of luteolin was attempted using two glucansucrases: the dextransucrase from Leuconostoc mesenteroides NRRL B-512F and the alternansucrase from L. mesenteroides NRRL B-23192. Reactions were carried out in aqueous-organic solvents to improve luteolin solubility. A molar conversion of 44% was achieved after 24h of reaction catalysed by dextransucrase from L. mesenteroides NRRL B-512F in a mixture of acetate buffer (70%)/bis(2-methoxyethyl) ether (30%). Two products were characterised by nuclear magnetic resonance (NMR) spectroscopy: luteolin-3'-O-alpha-d-glucopyranoside and luteolin-4'-O-alpha-d-glucopyranoside. In the presence of alternansucrase from L. mesenteroides NRRL B-23192, three additional products were obtained with a luteolin conversion of 8%. Both enzymes were also able to glucosylate quercetin and myricetin with conversion of 4% and 49%, respectively.
来自肠膜明串珠菌NRRL B - 512F的葡聚糖蔗糖酶和来自肠膜明串珠菌NRRL B - 23192的交替蔗糖酶。反应在水 - 有机溶剂中进行以提高木犀草素的溶解度。在由肠膜明串珠菌NRRL B - 512F的葡聚糖蔗糖酶催化的反应中，于醋酸盐缓冲液（70%）/双（2 - 甲氧基乙基）醚（30%）的混合物中反应24小时后，实现了44%的摩尔转化率。通过核磁共振（NMR）光谱对两种产物进行了表征：木犀草素 - 3'-O-α - D - 吡喃葡萄糖苷和木犀草素 - 4'-O-α - D - 吡喃葡萄糖苷。在存在来自肠膜明串珠菌NRRL B - 23192的交替蔗糖酶的情况下，获得了另外三种产物，木犀草素转化率为8%。两种酶也能够分别以4%和49%的转化率对槲皮素和杨梅素进行糖基化反应。
