Suzuki N, Urata M, Miyauchi T, Wakisaka M, Shimazaki J, Hosoya T
Endocrinol Jpn. 1983 Oct;30(5):657-61. doi: 10.1507/endocrj1954.30.657.
By serial transplantation of CS 1, a subline of Shionogi carcinoma SC 115, to female mice, another subline was obtained and designated CS2. The subline showed a complete loss of androgen dependency on the growth of the tumor. When male mice bearing the tumor were castrated and treated with testosterone, the activity of RNA polymerase I in isolated nuclei from the tumor hardly varied during the period of the experiments (36 h), while the activity of RNA polymerase II exhibited a transient increase (about 40%) at 6 h after the testosterone injection. The results, together with the previous ones showing 80% and 40% increases in RNA polymerase I activity at 24 h after testosterone administration in the case of SC 115 (androgen-dependent tumor) and CS 1 (less androgen-dependent tumor), respectively, indicate that the stimulation of RNA polymerase I activity by androgen in the tumor tissues is closely related to the androgen dependency on the growth of the tumors.
通过将盐野义癌SC 115的一个亚系CS 1连续移植到雌性小鼠体内，获得了另一个亚系并命名为CS2。该亚系显示出肿瘤生长对雄激素的依赖性完全丧失。当对患有该肿瘤的雄性小鼠进行阉割并用睾酮治疗时，在实验期间（36小时），从肿瘤中分离出的细胞核中RNA聚合酶I的活性几乎没有变化，而RNA聚合酶II的活性在注射睾酮后6小时出现短暂增加（约40%）。这些结果，连同之前的结果，即在SC 115（雄激素依赖性肿瘤）和CS 1（雄激素依赖性较小的肿瘤）的情况下，睾酮给药后24小时RNA聚合酶I活性分别增加80%和40%，表明雄激素对肿瘤组织中RNA聚合酶I活性的刺激与肿瘤生长对雄激素的依赖性密切相关。
