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[细胞中叶绿素a的荧光光谱和吸收光谱分解为其组分]

[Resolution of the fluorescence and absorption spectra of chlorophyll a in the cell into its components].

作者信息

Litvin F F, Stadnichuk I N, Kruglov V P

出版信息

Biofizika. 1978 May-Jun;23(3):450-5.

PMID:667148
Abstract

The total number, band widths, absorption and fluorescence peak positions of universal forms of chlorophyll a in vivo were identified by means of computer curve analysis, Stepanov's relation and low temperature derivative spectroscopy. The identified pigment forms are as follows: Chl669(661), Chl676(674), Chl686(680), Chl691(687), Chl697(691), Chl706(698), Chl715(702) Chl726(709), Chl738(715) and Chl750--755(720--724) (the lower index--absorption peak position, the upper one--fluorescence peak position). The O--O electronic transitions vibronic bands energy one of the forms of chlorphyll alpha enumerated above changes for constant equal 140 cm--1.

摘要

通过计算机曲线分析、斯捷潘诺夫关系和低温导数光谱法,确定了体内叶绿素a通用形式的总数、带宽、吸收和荧光峰位置。所确定的色素形式如下:Chl669(661)、Chl676(674)、Chl686(680)、Chl691(687)、Chl697(691)、Chl706(698)、Chl715(702)、Chl726(709)、Chl738(715)和Chl750 - 755(720 - 724)(下标为吸收峰位置,上标为荧光峰位置)。上述叶绿素α形式之一的O - O电子跃迁振动带能量变化恒定为140cm⁻¹。

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