Dierich A, Wintzerith M, Ciesielski-Treska J, Mandel P
Biochimie. 1978;60(2):137-46. doi: 10.1016/s0300-9084(78)80746-9.
RNA metabolism of M1 adrenergic neuroblastoma cells was investigated in logarithmic proliferating, stationary and differentiated states. [3h] uridine labelling experiments showed that in the stationary phase (10 days of culture) the cells incorporated less radioactive precursors into RNA than in the logarithmic phase (3 days of culture). Cells differentiated by bromodeoxyuridine treatment were compared with proliferating cells. At short labelling time, the BrdU-treated cells incorporated more [3H] uridine in RNA than the controls. But when morphological differentiation became irreversible, the treated cells incorporated less [3H] uridine into RNA. Our results show that morphological differentiation by BrdU-treatment of neuroblastoma M1 cells is not accompanied by striking changes in RNA metabolism.
在对数增殖、静止和分化状态下研究了M1肾上腺素能神经母细胞瘤细胞的RNA代谢。[3H]尿苷标记实验表明，在静止期（培养10天），细胞掺入RNA的放射性前体比对数期（培养3天）少。将经溴脱氧尿苷处理分化的细胞与增殖细胞进行比较。在短标记时间，经BrdU处理的细胞在RNA中掺入的[3H]尿苷比对照多。但当形态分化变得不可逆时，处理过的细胞在RNA中掺入的[3H]尿苷减少。我们的结果表明，用BrdU处理神经母细胞瘤M1细胞导致的形态分化并没有伴随着RNA代谢的显著变化。
