Lange K, Herken R, Keller K, Herken H
Naunyn Schmiedebergs Arch Pharmacol. 1977 Jul;298(3):259-62. doi: 10.1007/BF00500897.
Vital staining of neuroblastoma cells with acridine orange produces a bright intracellular red-orange fluorescence most probably due to the occurrence of RNA. The distribution of this fluorescence depends on the state of morphological differentiation. The fluorescence is predominantly found in the perikaryon, the growth cones, and the endings of the processes of differentiated cells. This is of special interest in respect to the biochemistry of differentiation and the function of nerve cells. Comparative autoradiographical studies with 3H-uridine demonstrate that the newly synthesised RNA is transported into the endings of the cell processes.
用吖啶橙对神经母细胞瘤细胞进行活体染色会产生明亮的细胞内红橙色荧光，这很可能是由于RNA的存在。这种荧光的分布取决于形态分化状态。荧光主要出现在分化细胞的胞体、生长锥和突起末端。这对于分化的生物化学和神经细胞的功能具有特殊意义。用3H-尿苷进行的比较放射自显影研究表明，新合成的RNA被转运到细胞突起的末端。
