Veeger C, Laane C, Scherings G, van Zeeland Wolbers L
Biochimie. 1978;60(3):237-43. doi: 10.1016/s0300-9084(78)80820-7.
Nitrogen fixation in A. vinelandii and R. leguminosarum bacteroides shows identical characteristics with respect to the dependence on membrane energization, the sensitivity to uncouplers, the ATP/ADP-ratio, and the dependences on flavodoxinhydroquinone as electrondonor. Although we have been successful in preparing inside-out vesicles which can be energized, attempts to couple these membranes to N2-ase were still unsuccessful. One of the major problems could be the failure to energize these vesicles directly by ATP. Although subject to polymerisation after addition of MgCl2, it could be shown that the actual mol.wt. of the O2-stable N2-ase complex is about 300,000 in agreement with a 1:1:1 stoichiometry of the three constituent proteins, namely, component I, component II and the 2Fe-2S protein.
在棕色固氮菌和根瘤菌的类菌体中，固氮作用在依赖膜能量化、对解偶联剂的敏感性、ATP/ADP比率以及对黄素氧还蛋白氢醌作为电子供体的依赖性方面表现出相同的特征。尽管我们成功制备了能够被能量化的内翻囊泡，但尝试将这些膜与固氮酶偶联仍未成功。其中一个主要问题可能是无法通过ATP直接为这些囊泡提供能量。尽管在添加MgCl₂后会发生聚合反应，但可以证明，O₂稳定的固氮酶复合物的实际分子量约为300,000，这与三种组成蛋白（即组分I、组分II和2Fe-2S蛋白）的1:1:1化学计量比一致。
