Kawashima S, Imahori K
J Biochem. 1983 Dec;94(6):1781-7. doi: 10.1093/oxfordjournals.jbchem.a134529.
DNA-histone complexes were reconstituted from DNA and acid-extracted core histones and the products were characterized by micrococcal nuclease digestion to examine whether proper nucleosome structure had been reconstituted. No nucleosome structure was produced starting from the mixture of acid-extracted histones and purified DNA in 2 M NaCl-5 M urea, while the reassociation of chromatin by the same procedures was successful. This was due to the inappropriate conformation of acid-extracted histones, which was preserved in 2 M NaCl even in the presence of 5 M urea. If acid-extracted histones were reannealed from the completely denatured state, such as in 5 M urea, 6 M guanidine hydrochloride or 0.6 M NaCl-5 M urea, reconstitution of nucleosome structure was always successful.
从DNA和酸提取的核心组蛋白中重建DNA-组蛋白复合物，并通过微球菌核酸酶消化对产物进行表征，以检查是否已重建出合适的核小体结构。在2M NaCl-5M尿素中，从酸提取的组蛋白和纯化的DNA混合物开始，未产生核小体结构，而通过相同程序进行染色质的重新缔合则是成功的。这是由于酸提取的组蛋白构象不合适，即使在存在5M尿素的情况下，该构象仍在2M NaCl中得以保留。如果酸提取的组蛋白从完全变性的状态进行复性，例如在5M尿素、6M盐酸胍或0.6M NaCl-5M尿素中，核小体结构的重建总是成功的。
