Guillaume G, Szymanowicz G, Randoux A, Borel J P
Biomedicine. 1978 Feb;29(1):5-6.
After extraction of 1% of the total collagen of rat skin by 150 mM NaCl, a 1% SDS solution extracts 80% of the total collagen, while the residue is extracted by a mixture of beta mercapto-ethanol and SDS. These extractions, which respect the peptide linkages, cleave the allysyl cross-links. They provide an insight on the composition of some insoluble collagen molecules of the skin and on the importance of disulfide bridges in their architecture.
用150 mM氯化钠提取大鼠皮肤总胶原蛋白的1%后，1%十二烷基硫酸钠（SDS）溶液可提取总胶原蛋白的80%，而残留物则用β-巯基乙醇和SDS的混合物提取。这些提取过程在尊重肽键的同时，会切断烯丙基交联键。它们有助于深入了解皮肤中一些不溶性胶原蛋白分子的组成以及二硫键在其结构中的重要性。
