Liguori L, Campione O, Marchesini G, Roversi C A, Vatrella S, Melli A, Greco V, Marrano D
Arch Sci Med (Torino). 1983 Oct-Dec;140(4):405-11.
Effectiveness of surgically induced acute hepatic failure in pig and most suitable time to apply artificial support in hepatic coma are evaluated in this work. Five male pigs weighing about 30-35 kg are employed. Latero-lateral porto-caval shunt was performed; the vascular disconnection of liver was obtained by ligature of blood vessels. Ligature was also placed on main biliary way after cholecistectomy. Blood samples were obtained (at 0, 1, 2, 6, 12, 18, 24 hours) to essay serum bilirubin, alkaline phosphatase and GOT-GPT levels as index of cholestasis and necrosis. Porto-caval encephalopathy was evaluated by means of serum ammonium levels, aminoacid pattern and E.E.G. Serum aminoacid pattern was carefully determined; its changes were found similar in man during coma. All pigs died 24-36 hours after surgery with liver ischemic and necrosis. Clinical and laboratory data obtained in experimental conditions were found similar to picture of acute hepatic failure in man, confirming validity of our model.
本研究评估了手术诱导的猪急性肝衰竭的有效性以及在肝昏迷中应用人工支持的最合适时间。选用了5头体重约30 - 35千克的雄性猪。进行了侧侧门腔静脉分流术；通过结扎血管实现肝脏血管离断。胆囊切除术后也对主要胆管进行了结扎。（在0、1、2、6、12、18、24小时）采集血样以检测血清胆红素、碱性磷酸酶以及谷草转氨酶 - 谷丙转氨酶水平，作为胆汁淤积和坏死的指标。通过血清铵水平、氨基酸模式和脑电图评估门腔静脉性脑病。仔细测定血清氨基酸模式；发现其变化与人昏迷时相似。所有猪在手术后24 - 36小时因肝脏缺血和坏死死亡。实验条件下获得的临床和实验室数据与人急性肝衰竭的情况相似，证实了我们模型的有效性。
