Romquin N, Plouet J, Barritault D, Courtois Y
Biol Cell. 1983;49(2):109-18. doi: 10.1111/j.1768-322x.1984.tb00228.x.
Human and bovine vascular endothelial cells from the umbilical vein and the aorta, respectively, were cultured in the presence of EDGF (a growth factor prepared from bovine retina) on plastic or on extracellular matrix (ECM). Both EDGF and ECM are required to allow the maximal proliferation of human cells and their organization in a typical monolayer. Conversely, bovine aortic endothelial cells grow perfectly in the absence of both factors in 6% fetal calf serum. However, a requirement for EDGF can also be demonstrated in low serum conditions, or in cells at high passage number. ECM had no growth promoting activity by itself. Thrombin acts similarly to EDGF on bovine serum-starved cells. EDGF prolongs the in vitro lifespan of both types of cells. Cells at all stages still synthesize factor VIII antigen as revealed by immunofluorescence. Thus EDGF, like other growth factors from brain, FGF or ECGF, may have an important role in angiogenesis, a critical problem in pathological retinas.
分别从人脐带静脉和牛主动脉获取的人源及牛源血管内皮细胞，在塑料培养皿或细胞外基质（ECM）上，于EDGF（一种从牛视网膜制备的生长因子）存在的条件下进行培养。EDGF和ECM对于人细胞的最大增殖及其在典型单层中的组织排列都是必需的。相反，牛主动脉内皮细胞在不含这两种因子但含有6%胎牛血清的条件下能完美生长。然而，在低血清条件下或高传代次数的细胞中，也能证明对EDGF的需求。ECM自身没有促生长活性。凝血酶对牛血清饥饿细胞的作用与EDGF类似。EDGF可延长这两种细胞的体外寿命。免疫荧光显示，处于各个阶段的细胞仍能合成因子VIII抗原。因此，EDGF与来自脑的其他生长因子（如FGF或ECGF）一样，可能在血管生成中发挥重要作用，而血管生成是病理性视网膜中的一个关键问题。
