Ohtomo T
Can J Microbiol. 1983 Dec;29(12):1653-60. doi: 10.1139/m83-253.
In a previous paper, we showed that bile acid derivatives inhibit capsule formation as well as taurine biosynthesis in a taurine+ (Tau+) encapsulated strain of Staphylococcus aureus. In the present study, binding of [14C]cholic acid [( 14C]CA) and [14C]taurocholic acid [( 14C]TA) to the staphylococcal polysaccharide antigen (SPA) of the capsular fraction was examined. The bile acids were found to bind with SPA via taurine of the Tau+ cells. [14C]CA bound with the SPA fraction of the Tau+ strain within 10-30 min, whereas 60-120 min was required in the binding of [14C]TA. Various bile acids competed with cholic acid binding to Tau+ cells which was shown by the inhibition of binding with cholic acid or taurocholic acid but not with glycholic acid. Binding of bile acid derivatives to a Tau- encapsulated mutant or to capsular material from this mutant was not observed.
在之前的一篇论文中，我们表明胆汁酸衍生物可抑制金黄色葡萄球菌的一个牛磺酸阳性（Tau+）被膜菌株中的荚膜形成以及牛磺酸生物合成。在本研究中，检测了[14C]胆酸[（14C）CA]和[14C]牛磺胆酸[（14C）TA]与荚膜部分的葡萄球菌多糖抗原（SPA）的结合情况。发现胆汁酸通过Tau+细胞的牛磺酸与SPA结合。[14C]CA在10 - 30分钟内与Tau+菌株的SPA部分结合，而[14C]TA的结合则需要60 - 120分钟。各种胆汁酸与胆酸竞争与Tau+细胞的结合，这通过对胆酸或牛磺胆酸结合的抑制得以体现，但对甘胆酸的结合无抑制作用。未观察到胆汁酸衍生物与一个Tau - 被膜突变体或该突变体的荚膜物质的结合。
