Genin C, Lesavre P
Mol Immunol. 1983 Oct;20(10):1069-72. doi: 10.1016/0161-5890(83)90114-1.
Insoluble immune-complexes (IC) are solubilized by complement. A functional alternative pathway (AP) is required for solubilization. Classical pathway (CP) has an enhancing effect, but the effect of antigen-antibody (Ag-Ab) ratio and the relative role of AP and CP at these different Ag-Ab ratios is controversial. We reinvestigated these factors in a human model (IC prepared with tetanus toxoid and affinity purified human Ab solubilized by normal serum--NHS). We present evidence that (1) IC prepared at Ag excess are only partially solubilized. (2) IC prepared at large Ag excess are not solubilized by AP. (3) AP prepared at Ab excess are solubilized exclusively by AP. (4) IC prepared at equivalence are solubilized by AP and CP acting in synergy.
不溶性免疫复合物（IC）可被补体溶解。溶解需要功能性替代途径（AP）。经典途径（CP）有增强作用，但抗原抗体（Ag-Ab）比例的影响以及在这些不同Ag-Ab比例下AP和CP的相对作用存在争议。我们在一个人体模型中重新研究了这些因素（用破伤风类毒素和经亲和纯化的人抗体制备的IC，由正常血清——NHS溶解）。我们提供的证据表明：（1）在抗原过量时制备的IC仅部分溶解。（2）在大量抗原过量时制备的IC不能被AP溶解。（3）在抗体过量时制备的IC仅由AP溶解。（4）在等价时制备的IC通过AP和CP协同作用而溶解。
