羧肽酶A催化的肽和酯水解反应的激活与抑制机制。

Mechanisms for activation and inhibition of carboxypeptidase A catalyzed hydrolyses of peptides and esters.

作者信息

Sebastian J F, Lo W Y

出版信息

Can J Biochem. 1978 May;56(5):329-33. doi: 10.1139/o78-051.

Abstract

3,3-Diphenylpropanoate (DPP) activates the carboxypeptidase A catalyzed hydrolysis of benzoylglycyl-L-phenylalanine (BzGly-L-Phe) (Ka = 2.1 x 10 (-3) M) and inhibits ester hydrolysis uncompetitively (K1 =2.1 X 10 (-3) M). A common modifier binding site located adjacent to the peptide and ester substrate binding sites is proposed. The forms of the pathways proposed for activation and inhibition are remarkably similar.

摘要

3,3-二苯基丙酸酯（DPP）可激活羧肽酶A催化的苯甲酰甘氨酰-L-苯丙氨酸（BzGly-L-Phe）的水解反应（Ka = 2.1×10⁻³ M），并以非竞争性方式抑制酯水解反应（K1 = 2.1×10⁻³ M）。研究提出，在肽和酯底物结合位点附近存在一个共同的修饰剂结合位点。所提出的激活和抑制途径形式非常相似。