Hanzlíková V, Gutmann E
Cell Tissue Res. 1978 May 18;189(1):155-66. doi: 10.1007/BF00223126.
The ultrastructure of the neuromuscular junction (n.m.j.) of the androgen-sensitive levator ani muscle was studied in normal adult male rats, in 8-month-old rats castrated at the age of one month and in castrated rats treated with testosterone propionate (TP). Castration does not result in significant changes of the n.m.j. The density of synaptic vesicles and the postsynaptic junctional folds remain practically normal inspite of marked atrophy of the muscle. TP administration for 7 days results in marked changes in pre- and postsynaptic structures. There is slow progressive depletion of synaptic vesicles, appearance of cisternae and coated vesicles in axon terminals, and coalescence of coated vesicles with the plasma membrane. Coated vesicles are also found inside Schwann cells and among junctional folds. Dense core vesicles appear both in the axon terminals and in the postsynaptic area. Collateral sprouting of terminal axons with the formation of new immature junctions is observed. After 35 days of TP administration depletion of synaptic vesicles continues. Glycogen beta-particles, mostly freely dispersed, occasionally seen in axon terminals 7 days after TP administration, subsequently increase in number. In the endplate zone of the muscle fibre increased protein synthesis is indicated by a rapid increase in ribosomes and irregularly located myofilaments and myofibrils. The appearance of n.m.j. after testosterone administration resembles that described after nerve stimulation; the degree of change is however less pronounced.
在正常成年雄性大鼠、1月龄时阉割的8月龄大鼠以及用丙酸睾酮（TP）治疗的阉割大鼠中，研究了雄激素敏感的提肛肌神经肌肉接头（n.m.j.）的超微结构。阉割不会导致神经肌肉接头发生显著变化。尽管肌肉明显萎缩，但突触小泡的密度和突触后连接褶皱实际上仍保持正常。给予TP 7天会导致突触前和突触后结构发生显著变化。突触小泡缓慢进行性耗竭，轴突终末出现池和被膜小泡，且被膜小泡与质膜融合。在施万细胞内和连接褶皱之间也发现了被膜小泡。致密核心小泡出现在轴突终末和突触后区域。观察到终末轴突发侧支芽生并形成新的未成熟接头。给予TP 35天后，突触小泡的耗竭仍在继续。糖原β颗粒大多自由分散，在给予TP 7天后偶尔可见于轴突终末，随后数量增加。在肌纤维的终板区，核糖体、不规则排列的肌丝和肌原纤维数量迅速增加，表明蛋白质合成增加。给予睾酮后神经肌肉接头的外观类似于神经刺激后所描述的；然而，变化程度不太明显。
