Dearden L C, Mosier H D, Espinosa T
Cell Tissue Res. 1978 May 18;189(1):67-89. doi: 10.1007/BF00223122.
Cortisone-treated Buffalo rats have been parabiosed with untreated controls of the same age. The optical and electron microscopy, including histochemistry, of costal cartilage of these rats has been compared with that in single cortisone treated rats, single controls, and control parabiosed with control rats, at 14 and 28 days after parabiosis. Single cortisone-treated rats, in comparison to controls, have shown the greatest alteration in cellular morphology and in the extracellular matrix both at 14 and at 28 days. Cortisone-treated parabiosed rats demonstrate a gradation of these alterations. Cellular alterations include enhancement of lipid and glycogen deposition concrurently with the presence of numerous large cytoplasmic vacuoles containing beaded irregularly-shaped filaments, banded or unbanded collagen-like fibrils, and/or electron dense lamellar bodies. In the extracellular matrix, matrix vesicles, amianthoid fibers, randomly oriented unbanded fibrillar materials, and filament-like materials are most prominent in the single cortisone-treated rats and they are progressively less prominent in the cortisone-treated parabiosed rats, and in the parabiosed and single controls. Calcification of the extracellular matrix follows a similar pattern and is observed initially in pericellular halos of the single cortisone and in cortisone-treated rats parabiosed with controls. Histochemical techniques have shown that chondroitin sulfate is less demonstrable in the single cortisone and in the cortisone-treated parabiosed rats than it is in the single or parabiosed controls at 14 days but, at 28 days, all untreated or treated rats, single or parabiosed are basically comparable. Glycoproteins are prominent in the single cortisone-treated rats both at 14 and at 28 days and, at these same times, they are progressively less prominent in the cortisone-treated parabiosed rats and in the single or parabiosed controls. Many of the cortisone induced alterations in costal cartilage are suggestive of enhancement of the aging process.
已将经可的松处理的布法罗大鼠与相同年龄的未处理对照大鼠进行联体。在联体后14天和28天，对这些大鼠肋软骨进行了光学显微镜和电子显微镜检查，包括组织化学检查，并与单次接受可的松处理的大鼠、单次对照大鼠以及与对照大鼠联体的对照大鼠进行了比较。与对照相比，单次接受可的松处理的大鼠在14天和28天时细胞形态和细胞外基质的改变最大。经可的松处理的联体大鼠表现出这些改变的程度不同。细胞改变包括脂质和糖原沉积增加，同时存在许多大的细胞质空泡，其中含有串珠状不规则形状的细丝、带状或非带状胶原样纤维和/或电子致密层状体。在细胞外基质中，基质小泡、石棉样纤维、随机取向的非带状纤维状物质和丝状物质在单次接受可的松处理的大鼠中最为突出，在经可的松处理的联体大鼠以及联体和单次对照大鼠中逐渐不那么突出。细胞外基质的钙化遵循类似模式，最初在单次接受可的松处理的大鼠以及与对照联体的经可的松处理的大鼠的细胞周围晕中观察到。组织化学技术表明，在14天时，硫酸软骨素在单次接受可的松处理的大鼠以及经可的松处理的联体大鼠中比在单次或联体对照大鼠中更难检测到，但在28天时，所有未处理或处理过的大鼠，无论是单次还是联体，基本相当。糖蛋白在单次接受可的松处理的大鼠中在14天和28天时都很突出，在这些相同时间，它们在经可的松处理的联体大鼠以及单次或联体对照大鼠中逐渐不那么突出。可的松诱导的肋软骨许多改变提示衰老过程增强。
