Dearden L C
Am J Anat. 1975 Nov;144(3):323-37. doi: 10.1002/aja.1001440305.
The occurrence of intracellular fibrillar material (frequently banded) has been studied in normal costal and tracheal chondrocytes of rats at various ages ranging from 1 to 90 days. The study methods have included digestion with collagenase, electron histochemical techniques and routine electron microscopy. Banded fibrillar material has been observed intracellularly in vesicles or in electron-dense bodies in perichondrial and subperichondrial chondrocytes from rats of all ages. These fibrils and extracellular collagen fibrils are partially and equally degradable by collagenase, they are positive after staining with phosphotungstic acid or with silver nitrate methenamine, and their lucency corresponds with that of collagen when they are stained only with lead citrate. They have not been observed in intracellular clefts. They, therefore, seem to be formed intracellularly and to be exocytosed subsequently. Large vesicles and electron-dense bodies seem to be derived from Golgi saccules. A mechanism whereby banded intracellular fibrils could be formed from tropocollagen molecules is postulated. The frequency of occurrence and the diameter of intracellular fibrils seems to increase with increasing age.
对1至90日龄大鼠正常肋软骨和气管软骨细胞内细胞内纤维状物质（常呈带状）的出现情况进行了研究。研究方法包括用胶原酶消化、电子组织化学技术和常规电子显微镜检查。在所有年龄段大鼠的软骨膜和软骨膜下软骨细胞的囊泡或电子致密小体中均观察到细胞内带状纤维状物质。这些纤维与细胞外胶原纤维可被胶原酶部分且同等程度地降解，用磷钨酸或硝酸银六亚甲基四胺染色后呈阳性，仅用枸橼酸铅染色时其透明度与胶原的透明度一致。在细胞内裂隙中未观察到它们。因此，它们似乎是在细胞内形成并随后被胞吐出来的。大囊泡和电子致密小体似乎源自高尔基体囊泡。推测了一种由原胶原蛋白分子形成细胞内带状纤维的机制。细胞内纤维的出现频率和直径似乎随年龄增长而增加。
